Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Srebf2

Cell type

Cell type Class
Others
Cell type
NLPCs
NA
NA

Attributes by original data submitter

Sample

source_name
Neural stem cell-derived lens progenitor-like cells (NLPCs)
cell type
Neural stem cell-derived lens progenitor-like cells (NLPCs) differentiated from neural stem cells
passages
10
strain
FvB/C57BL6 hybrid
chip antibody
Srebp2 (10007663, Cayman Chemical)
genotype
wild type

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked, sonicated and then incubated with Qki-5, Pol II, and Srebp2 antibodies. Protein-DNA complex were washed and ChIP'ed DNA were isolated for library preparation. Libraries were prepared by KAPA Hyper Prep Kit (KK8504, Kapa Biosystems).

Sequencing Platform

instrument_model
HiSeq X Ten

mm10

Number of total reads
24895308
Reads aligned (%)
74.4
Duplicates removed (%)
40.0
Number of peaks
3711 (qval < 1E-05)

mm9

Number of total reads
24895308
Reads aligned (%)
74.4
Duplicates removed (%)
39.9
Number of peaks
3691 (qval < 1E-05)

Base call quality data from DBCLS SRA